Cytotoxicity Test ISO 10993-5: In vitro cytotoxicity test

Cytotoxicity is an in vitro test to determine whether the toxic effects of medical devices interacting with the body, by indirect or direct contact, will cause any cell death It is aimed to determine the toxic effect on mammalian cells, depending on the duration and concentration of the test sample. It includes Neutral Red Uptake (NKA), MTT and XTT tests performed within the scope of cytotoxicity. In our center, the cytotoxicity test is performed by the MTT test, which is based on the principle of reducing the succinate dehydrogenesis enzyme in mitochondria to purple colored formazone crystals. It is implemented according to the ISO 10993-5 standard.